Defatted mung bean meal was hydrolyzed with bromelain to obtain MPH, which was fractionated into different peptide fractions (<1 kDa, 1–5 kDa, 5–10 kDa, >10 kDa) using membrane ultrafiltration. The <1 kDa was the most active inhibitor of angiotensin converting enzyme (ACE) and renin. Five peptides (LPRL, YADLVE, LRLESF, HLNVVHEN, and PGSGCAGTDL) identified from the <1 kDa fraction were studied for antihypertensive effects in comparison to the MPH. Results showed that LRLESF was the most potent inhibitor of ACE (IC₅₀ = 5.4 µM) while LPRL was the weakest (IC₅₀ = 1912 µM). In contrast, YADLVE was the strongest renin inhibitor with 97% inhibition while LRLESF was the weakest (~30%). Oral administration to spontaneously hypertensive rats revealed strong blood pressure reductions with up to −36 mmHg when compared to −15 mmHg for MPH. However, YADLVE had the most persistent effect after 24 h while PGSGCAGTDL had the least.